Gotaq Green Master Mix Ingredients

New formulation of taq dna polymerase with enhanced performance over standard taq dna polymerase.

Gotaq green master mix ingredients. Gotaq green master mix contains two dyes blue and yellow that allow monitoring of progress during electrophoresis. High performance taq dna polymerase nucleotides dntps buffers and master mixes provide increased reliability and consistency for routine endpoint pcr. Vortex the master mix then spin it briefly in a microcentrifuge to collect the material at the bottom of the tube. Gotaq green master mix 2x 12 5µl 1x.

Gotaq g2 is a full length recombinant taq polymerase supplied with buffers designed for enhanced amplification. A protocol for a stabilized 2x master mix that includes all components for quantitative pcr except sample dna primers and water. Component volume final conc. Fyi the amplification efficiency for all primers have been validated using the commercial sybr green master mix and we re running our samples on a cfx96 real time detection system bio rad.

Gotaq qpcr master mixはready to useの安定した2倍濃度の試薬であり gotaq hot start polymerase mgcl 2 dntps bryt green dye 反応バッファーからなります マスターミックスには低レベルのcarboxy x rhodamine cxr が含まれますが さらに別のチューブにてcxrが付属します. Gotaq products offer a choice of taq polymerase formulations for basic pcr hot start pcr and long range pcr. Product contents gotaq green master mix cat size m7121 10 reactions m7122 100 reactions m7123 1 000 reactions includes gotaq green master mix 2x and nuclease free water. The green master mix allows direct gel analysis after amplification.

Thaw the gotaq green master mix at room temperature. Available in two all in one master mixes. Thermo scientific dreamtaq green pcr master mix 2x is a ready to use solution containing dreamtaq dna polymerase optimized dreamtaq green buffer mgcl 2 and dntps. Gotaq green master mix is a premixed ready to use solution containing bacterially derived taq dna polymerase dntps mgcl2 and reaction buffers at optimal concentrations for efficient amplification of.

Prepare one of the following reaction mixes on ice. Gotaq green master mix is a premixed ready to use solution containing bacterially derived taq dna polymerase dntps mgcl 2 and reaction buffers at optimal concentrations for efficient amplification of dna templates by pcr. It is capable of robust amplification of up to 6 kb from genomic dna and up to 20 kb from viral dna. The master mix retains all features of dreamtaq dna polymerase.

For a 25µl reaction volume.